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fdft1  (Proteintech)


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    Structured Review

    Proteintech fdft1
    Comparative proteomic analysis of whole mouse livers and iHLCs (A) Venn diagram depicting overlap of significant DE proteins in APOE4 vs. APOE3 across whole mouse livers and iHLCs (batch 1 and 2). (B) Gene Ontology Biological Process (GO-BP) and KEGG pathway analysis of the 70 proteins shared across mouse liver and iHLCs, showing protein counts per category. (C) Heatmap of proteins consistently upregulated or downregulated in APOE4 vs. APOE3 across whole mouse liver and iHLCs (batch 1 and 2). (D–E) Western blot analysis of CYP27A1, <t>FDFT1,</t> and PGAM1 in APOE4 and APOE3 whole mouse livers, with relative densitometry quantification. n = 16 per group. (F–G) Western blot analysis of CYP27A1, FDFT1, and PGAM1 in APOE4 and APOE3 isogenic iHLCs, with relative densitometry quantification. n = 6 per group. (E and G) Statistical significance was determined by unpaired t test. Data are shown as mean ± SD. ∗ p < 0.05.
    Fdft1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fdft1+antibody/FDFT1+Antibody/pmc12962117-429-19-20
    Average 93 stars, based on 31 article reviews
    fdft1 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "APOE4 drives widespread changes to the hepatic proteome and alters metabolic function"

    Article Title: APOE4 drives widespread changes to the hepatic proteome and alters metabolic function

    Journal: iScience

    doi: 10.1016/j.isci.2026.115035

    Comparative proteomic analysis of whole mouse livers and iHLCs (A) Venn diagram depicting overlap of significant DE proteins in APOE4 vs. APOE3 across whole mouse livers and iHLCs (batch 1 and 2). (B) Gene Ontology Biological Process (GO-BP) and KEGG pathway analysis of the 70 proteins shared across mouse liver and iHLCs, showing protein counts per category. (C) Heatmap of proteins consistently upregulated or downregulated in APOE4 vs. APOE3 across whole mouse liver and iHLCs (batch 1 and 2). (D–E) Western blot analysis of CYP27A1, FDFT1, and PGAM1 in APOE4 and APOE3 whole mouse livers, with relative densitometry quantification. n = 16 per group. (F–G) Western blot analysis of CYP27A1, FDFT1, and PGAM1 in APOE4 and APOE3 isogenic iHLCs, with relative densitometry quantification. n = 6 per group. (E and G) Statistical significance was determined by unpaired t test. Data are shown as mean ± SD. ∗ p < 0.05.
    Figure Legend Snippet: Comparative proteomic analysis of whole mouse livers and iHLCs (A) Venn diagram depicting overlap of significant DE proteins in APOE4 vs. APOE3 across whole mouse livers and iHLCs (batch 1 and 2). (B) Gene Ontology Biological Process (GO-BP) and KEGG pathway analysis of the 70 proteins shared across mouse liver and iHLCs, showing protein counts per category. (C) Heatmap of proteins consistently upregulated or downregulated in APOE4 vs. APOE3 across whole mouse liver and iHLCs (batch 1 and 2). (D–E) Western blot analysis of CYP27A1, FDFT1, and PGAM1 in APOE4 and APOE3 whole mouse livers, with relative densitometry quantification. n = 16 per group. (F–G) Western blot analysis of CYP27A1, FDFT1, and PGAM1 in APOE4 and APOE3 isogenic iHLCs, with relative densitometry quantification. n = 6 per group. (E and G) Statistical significance was determined by unpaired t test. Data are shown as mean ± SD. ∗ p < 0.05.

    Techniques Used: Western Blot

    Related Articles

    FLAG-tag:

    Article Title: The compound LY295427 antagonizes 25-hydroxycholesterol through binding to INSIG.
    Article Snippet: .. Antibodies The primary antibodies used were as follows: mouse monoclonal antibody against β-actin (#A5441; Sigma), mouse monoclonal antibody against FLAG-tag (#F3165; Sigma), mouse monoclonal antibody against T7-tag (#69522; Merck), mouse monoclonal antibody against HA-tag (#H3663; Sigma), mouse monoclonal antibody P4D1 against ubiquitin (#SC-8017; Santa Cruz Biotechnology), mouse monoclonal antibody 9D5 against SCAP (#CRL-2347; ATCC), mouse monoclonal antibody 7D4 against SREBP2 (#CRL-2198; ATCC), anti-FDFT1 antibody (#13128; Proteintech), anti-LSS antibody (#18693; Proteintech), mouse monoclonal antibody 9E10 against MYC-tag, mouse monoclonal antibody A9 against HMGCR, mouse monoclonal antibody 1D2 against SREBP2, rabbit polyclonal antibody against EGFP-tag, rabbit polyclonal antibody against LDLR, and rabbit polyclonal antibody against HMGCR, which were prepared in our laboratory. .. The secondary antibodies used were as follows: peroxidase AffiniPure goat anti-mouse IgG (H+L) secondary antibody (#115-035-003; Jackson ImmunoResearch), peroxidase AffiniPure goat antirabbit IgG (H+L) secondary antibody (#111-035-144; Jackson ImmunoResearch), Alexa Fluor 555 donkey anti-mouse IgG (#A31570; Invitrogen), and mouse monoclonal antibody against GM130 (#610822; BD Biosciences).

    Ubiquitin Proteomics:

    Article Title: The compound LY295427 antagonizes 25-hydroxycholesterol through binding to INSIG.
    Article Snippet: .. Antibodies The primary antibodies used were as follows: mouse monoclonal antibody against β-actin (#A5441; Sigma), mouse monoclonal antibody against FLAG-tag (#F3165; Sigma), mouse monoclonal antibody against T7-tag (#69522; Merck), mouse monoclonal antibody against HA-tag (#H3663; Sigma), mouse monoclonal antibody P4D1 against ubiquitin (#SC-8017; Santa Cruz Biotechnology), mouse monoclonal antibody 9D5 against SCAP (#CRL-2347; ATCC), mouse monoclonal antibody 7D4 against SREBP2 (#CRL-2198; ATCC), anti-FDFT1 antibody (#13128; Proteintech), anti-LSS antibody (#18693; Proteintech), mouse monoclonal antibody 9E10 against MYC-tag, mouse monoclonal antibody A9 against HMGCR, mouse monoclonal antibody 1D2 against SREBP2, rabbit polyclonal antibody against EGFP-tag, rabbit polyclonal antibody against LDLR, and rabbit polyclonal antibody against HMGCR, which were prepared in our laboratory. .. The secondary antibodies used were as follows: peroxidase AffiniPure goat anti-mouse IgG (H+L) secondary antibody (#115-035-003; Jackson ImmunoResearch), peroxidase AffiniPure goat antirabbit IgG (H+L) secondary antibody (#111-035-144; Jackson ImmunoResearch), Alexa Fluor 555 donkey anti-mouse IgG (#A31570; Invitrogen), and mouse monoclonal antibody against GM130 (#610822; BD Biosciences).



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    Comparative proteomic analysis of whole mouse livers and iHLCs (A) Venn diagram depicting overlap of significant DE proteins in APOE4 vs. APOE3 across whole mouse livers and iHLCs (batch 1 and 2). (B) Gene Ontology Biological Process (GO-BP) and KEGG pathway analysis of the 70 proteins shared across mouse liver and iHLCs, showing protein counts per category. (C) Heatmap of proteins consistently upregulated or downregulated in APOE4 vs. APOE3 across whole mouse liver and iHLCs (batch 1 and 2). (D–E) Western blot analysis of CYP27A1, <t>FDFT1,</t> and PGAM1 in APOE4 and APOE3 whole mouse livers, with relative densitometry quantification. n = 16 per group. (F–G) Western blot analysis of CYP27A1, FDFT1, and PGAM1 in APOE4 and APOE3 isogenic iHLCs, with relative densitometry quantification. n = 6 per group. (E and G) Statistical significance was determined by unpaired t test. Data are shown as mean ± SD. ∗ p < 0.05.
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    Image Search Results


    Comparative proteomic analysis of whole mouse livers and iHLCs (A) Venn diagram depicting overlap of significant DE proteins in APOE4 vs. APOE3 across whole mouse livers and iHLCs (batch 1 and 2). (B) Gene Ontology Biological Process (GO-BP) and KEGG pathway analysis of the 70 proteins shared across mouse liver and iHLCs, showing protein counts per category. (C) Heatmap of proteins consistently upregulated or downregulated in APOE4 vs. APOE3 across whole mouse liver and iHLCs (batch 1 and 2). (D–E) Western blot analysis of CYP27A1, FDFT1, and PGAM1 in APOE4 and APOE3 whole mouse livers, with relative densitometry quantification. n = 16 per group. (F–G) Western blot analysis of CYP27A1, FDFT1, and PGAM1 in APOE4 and APOE3 isogenic iHLCs, with relative densitometry quantification. n = 6 per group. (E and G) Statistical significance was determined by unpaired t test. Data are shown as mean ± SD. ∗ p < 0.05.

    Journal: iScience

    Article Title: APOE4 drives widespread changes to the hepatic proteome and alters metabolic function

    doi: 10.1016/j.isci.2026.115035

    Figure Lengend Snippet: Comparative proteomic analysis of whole mouse livers and iHLCs (A) Venn diagram depicting overlap of significant DE proteins in APOE4 vs. APOE3 across whole mouse livers and iHLCs (batch 1 and 2). (B) Gene Ontology Biological Process (GO-BP) and KEGG pathway analysis of the 70 proteins shared across mouse liver and iHLCs, showing protein counts per category. (C) Heatmap of proteins consistently upregulated or downregulated in APOE4 vs. APOE3 across whole mouse liver and iHLCs (batch 1 and 2). (D–E) Western blot analysis of CYP27A1, FDFT1, and PGAM1 in APOE4 and APOE3 whole mouse livers, with relative densitometry quantification. n = 16 per group. (F–G) Western blot analysis of CYP27A1, FDFT1, and PGAM1 in APOE4 and APOE3 isogenic iHLCs, with relative densitometry quantification. n = 6 per group. (E and G) Statistical significance was determined by unpaired t test. Data are shown as mean ± SD. ∗ p < 0.05.

    Article Snippet: Primary antibodies included ALB (Abcam, ab207327), APOE (Abcam, ab183597), ASGR1 (Abcam, ab254261), CS (Cell Signaling, #14309), CYP27A1 (Abcam, ab126785), FDFT1 (Proteintech, 83020-1-RR), HDAC1 (Cell Signaling, #34589), HK1 (Cell Signaling, #2024), HNF4A (Abcam, ab92378), NANOG (Abcam, ab109250), OCT4 (Abcam, ab181557), PGAM1 (Cell Signaling, #12098), and SOX17 (Abcam, ab224637).

    Techniques: Western Blot